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Minocycline suppresses LPS-induced retinal microglia activation through downregulation of <t>TLR4</t> signaling. A : Real-time PCR analysis of Toll-like receptor (TLR) 2 and TLR4 mRNA expression in BV-2 microglial cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h (n=5 per group). *p<0.05 compared with the LPS treated with PBS group. B : Western blotting of TLR2 and TLR4 protein expression (n=4 per group).
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Minocycline suppresses LPS-induced retinal microglia activation through downregulation of <t>TLR4</t> signaling. A : Real-time PCR analysis of Toll-like receptor (TLR) 2 and TLR4 mRNA expression in BV-2 microglial cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h (n=5 per group). *p<0.05 compared with the LPS treated with PBS group. B : Western blotting of TLR2 and TLR4 protein expression (n=4 per group).
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Minocycline suppresses LPS-induced retinal microglia activation through downregulation of TLR4 signaling. A : Real-time PCR analysis of Toll-like receptor (TLR) 2 and TLR4 mRNA expression in BV-2 microglial cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h (n=5 per group). *p<0.05 compared with the LPS treated with PBS group. B : Western blotting of TLR2 and TLR4 protein expression (n=4 per group).

Journal: Molecular Vision

Article Title: The anti-inflammatory effect of minocycline on endotoxin-induced uveitis and retinal inflammation in rats

doi:

Figure Lengend Snippet: Minocycline suppresses LPS-induced retinal microglia activation through downregulation of TLR4 signaling. A : Real-time PCR analysis of Toll-like receptor (TLR) 2 and TLR4 mRNA expression in BV-2 microglial cells exposed to 100 ng/ml of lipopolysaccharide (LPS) with or without minocycline (50 μg/ml) for 6 h (n=5 per group). *p<0.05 compared with the LPS treated with PBS group. B : Western blotting of TLR2 and TLR4 protein expression (n=4 per group).

Article Snippet: After the electroblotting, the membranes were blocked with 5% non-fat milk in PBS with Tween 20 (PBST) for 1 h at RT, and then were incubated with primary antibodies against Toll-like receptor (TLR) 2 and TLR4 (1:1,000, Cell Signaling Technology) at 4 °C overnight. β-actin (Abcam) was used as an internal protein loading control.

Techniques: Activation Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot